# Green-synthesized zinc oxide nanoparticles using Echinops spinosus extract: A promising anticancer agent for hepatocellular carcinoma

## TL;DR

This study shows that green-synthesized zinc oxide nanoparticles using Echinops spinosus extract are effective against liver cancer cells.

## Contribution

The paper introduces a sustainable method to synthesize ZnO nanoparticles with strong anticancer activity against hepatocellular carcinoma.

## Key findings

- ZnO nanoparticles showed 10.4-fold higher anti-proliferative effect on HepG2 cells compared to the extract.
- The IC50 for HepG2 cells was 19.94 µg/mL, indicating strong cytotoxicity.
- Green-synthesized ZnO nanoparticles have potential as eco-friendly anticancer agents.

## Abstract

The environmentally sustainable synthesis of nanoparticles has arisen as a viable alternative to traditional methods, tackling ecological and economic issues. This research investigates the green synthesis of ZnO nanoparticles utilizing an aqueous extract of Echinops Spinosus L. roots (ESRE), abundant in bioactive chemicals, as a natural reducing agent. The impacts of different quantities of precursors and reducing agents were methodically examined. The synthesized ZnO nanoparticles were characterized by zeta potential measurements (−15.2 mV), transmission electron microscopy (TEM), which indicated spherical and hexagonal morphologies with an average size of 20.47–48.22 nm, and energy-dispersive X-ray spectroscopy (EDX), confirming zinc and oxygen as the principal elements. Fourier transform infrared spectroscopy (FTIR) underscored plant-derived compounds’ contribution to nanoparticle stabilization. Cytotoxicity was assessed using the MTT assay on two cancer cell lines, HepG2 (hepatocellular carcinoma) and MCF-7 (breast cancer). The green-synthesized ZnO nanoparticles had substantial anticancer efficacy, with the ZnO nanoparticles exhibiting the most pronounced anti-proliferative effect on HepG2 cells (10.4 folds) and 5.4 folds more effective in MCF-7 cells compared to ESRE, with HepG2 cells IC50 value of 19.94 ± 0.11 µg/mL while the IC50 for MCF-7 cells was 75.65 ± 0.16 µg/mL. The results indicate that ZnO-NPs produced by green technologies exhibit significant potential as anticancer agents, especially for hepatocellular carcinoma treatment.