# Polyadic synapses introduce unique wiring architectures in T5 cells of Drosophila

## TL;DR

The study reveals how polyadic synapses create unique wiring patterns in T5 cells of fruit flies, influencing their motion-sensing network.

## Contribution

The paper identifies eight distinct polyadic synapse types connecting Tm and CT1 neurons to T5a dendrites and shows their directional wiring impact.

## Key findings

- Eight polyadic synapse types connect Tm and CT1 neurons to T5a dendrites.
- Different synapse types show distinct spatial patterns on T5a dendrites.
- Polyadic synapses establish directional wiring architecture in the T5 network.

## Abstract

Connectomes provide neuronal wiring diagrams and allow for investigating the detailed synaptic morphology of each connection. In the visual system of Drosophila, T5 cells are the primary motion-sensing neurons in the OFF-pathway and receive dendritic input from the excitatory Tm1, Tm2, Tm4, Tm9 and the inhibitory CT1 neurons. This connectivity, however, has not yet been investigated with respect to polyadic synapses known to be abundant in the fly nervous system. In this study, we use the FlyWire database and identify that Tm and CT1 cells wire on T5a dendrites via eight polyadic synapse types. We then explore the distribution of the different synapse types on T5a dendrites and find differences in their spatial patterns. Finally, we show that the polyadic morphology is setting a directional wiring architecture at the T5 network level. Our work showcases the directionality that polyadic synapses introduce in T5 connectivity.