# ZNF33B facilitates Japanese encephalitis virus replication by controlling HSPB1/8-mediated SUMOylation of nonstructural protein 5

## TL;DR

This study reveals how a human protein called ZNF33B helps the Japanese encephalitis virus replicate by stabilizing its genetic material and modifying a key viral protein.

## Contribution

The study identifies a novel role for ZNF33B in promoting JEV replication through SUMOylation of NS5, mediated by HSPB1/8.

## Key findings

- ZNF33B stabilizes JEV RNA and enhances replication by translocating to cytoplasmic viral replication complexes.
- ZNF33B promotes SUMOylation of NS5 at lysine residues 269 and 846, counteracting ubiquitination and degradation.
- HSPB1 and HSPB8 act as SUMO E3 ligases recruited by ZNF33B to facilitate NS5 SUMOylation.

## Abstract

Japanese encephalitis virus (JEV) is a significant flavivirus that poses a threat to public health, as it induces encephalitis in humans and reproductive disorders in sows. We have recently identified that zinc finger protein 33B (ZNF33B) is required for JEV infection by CRISPR-based functional genomic screening, yet the precise functions and mechanisms are not fully comprehended. In this study, ZNF33B was found to be involved in JEV infection, wherein it bound with JEV RNA to enhance its stability during replication. Additionally, ZNF33B underwent translocation from the nucleus to the cytoplasm to associate with viral replication complexes during JEV infection. Furthermore, ZNF33B stabilized JEV nonstructural protein 5 (NS5), rather than NS3, by inhibiting its polyubiquitination and promoting SUMOylation. The SUMOylation of JEV NS5 was found to compete with its ubiquitination at lysine residues 269 and 846. Through immunoprecipitation-mass spectrometry, we identified heat shock proteins HSPB1 and HSPB8 as potential mediators of the SUMOylation of JEV NS5. ZNF33B was shown to recruit HSPB1/8 to facilitate NS5 SUMOylation. Overall, our study highlighted the importance of ZNF33B in facilitating the SUMOylation of JEV NS5 through the recruitment of HSPB1 and HSPB8.

Japanese encephalitis virus (JEV) poses a severe global health threat, yet host factors regulating its replication remain poorly understood. Our study identifies ZNF33B as a critical host protein that enhances JEV replication by stabilizing viral RNA and facilitating SUMOylation of the viral polymerase NS5. We demonstrate that ZNF33B recruits HSPB1/8 as SUMO E3 ligases to modify NS5, thereby counteracting its polyubiquitination and proteasomal degradation. This SUMOylation-ubiquitination crosstalk at lysine residues 269 and 846 ensures NS5 stability, essential for viral replication. These findings unveil a novel mechanism by which JEV exploits host post-translational machinery to sustain replication. Targeting ZNF33B or viral SUMOylation could offer therapeutic strategies against JEV and related flaviviruses, with great significance for the development of antiviral interventions.