# The Expression and Clinical Significance of C1orf106 in Low‐Grade Serous Ovarian Cancer

## TL;DR

This study identifies C1orf106 as a potential diagnostic and prognostic marker for low-grade serous ovarian cancer.

## Contribution

The study introduces C1orf106 as a novel biomarker for low-grade serous ovarian cancer.

## Key findings

- C1orf106 is significantly overexpressed in LGSOC tissues compared to normal ovarian tissues.
- High C1orf106 expression correlates with better progression-free and overall survival in LGSOC patients.
- C1orf106 is associated with lower BMI and absence of residual disease in LGSOC patients.

## Abstract

Low‐grade serous ovarian cancer (LGSOC) is a rare subtype of ovarian cancer with distinct biological behavior. This study aimed to identify new biomarkers with potential diagnostic and prognostic value for LGSOC.

Gene‐expression data were downloaded from the Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified using R. Functional enrichment analyses were conducted to determine the biological functions and signaling pathways associated with DEGs. The mitogen‐activated protein kinase (MAPK) pathway‐related gene, chromosome 1 open reading frame 106 (C1orf106), was selected as the target gene. Immunohistochemistry and quantitative real‐time polymerase chain reaction (qRT‐PCR) were performed to verify its expression. Associations between C1orf106 expression and the clinical features of patients were analyzed using the chi‐square (χ
2) test. Prognostic significance was evaluated with survival analyses.

A total of 3099 upregulated and 4968 downregulated genes were identified in LGSOC. Gene set enrichment analysis (GSEA) demonstrated significant alterations in KRAS signaling and metabolic pathways between LGSOC and healthy controls. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses revealed enrichment in immune response and MAPK pathway alterations. Immunohistochemistry and qRT‐PCR confirmed that C1orf106 expression in LGSOC tissues was significantly higher than in normal ovarian tissues. Clinically, high C1orf106 expression was associated with lower BMI (< 25 kg/m2), the absence of visible residual disease, and improved progression‐free survival (PFS) and overall survival (OS) in univariate Cox and Kaplan–Meier analyses.

C1orf106 may serve as a promising marker for the diagnosis and prognosis of LGSOC.