Similar patterns of benzimidazole resistance alleles in ovine gastrointestinal nematodes from Western Canada and Eastern United States supports their shared origins and subsequent spread
Camila Queiroz, Michel Levy, Russell Avramenko, Rebecca Chen, Michaela Seal, Elizabeth Redman, Anne Zajac, John Stuart Gilleard
TL;DR
The study finds similar benzimidazole resistance patterns in sheep parasites from Canada and the US, suggesting shared origins and spread through animal movement.
Contribution
The study uses deep sequencing to compare resistance alleles in nematodes from two regions, supporting a model of resistance spread via livestock movement.
Findings
Benzimidazole resistance SNPs were at fixation for Haemonchus contortus in both regions.
Resistance SNPs for T. circumcincta and T. colubriformis were more prevalent in the USA than in Canada.
Similar major resistance alleles were found at similar frequencies in both regions, suggesting shared origins.
Abstract
Livestock movement facilitates translocation of anthelmintic resistant parasites, but the extent to which resistance emergence depends on animal movement is still poorly understood. Benzimidazole resistance is widespread in ovine trichostrongylid nematodes, and our understanding of its molecular basis now allows for molecular epidemiology investigations. This study applies deep amplicon sequencing of the isotype-1 β-tubulin locus to compare the prevalence and frequency of benzimidazole resistance Single Nucleotide Polymorphisms (SNPs), and their alleles, for trichostrongylid populations from 102 Western Canadian and 28 Eastern USA sheep flocks. For H. contortus, benzimidazole resistance SNPs were at fixation tin almost all flocks from both regions; that is, present at, or close to, 100 % frequency. For T. circumcincta and T. colubriformis, although at fixation in most Eastern USA…
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Taxonomy
TopicsHelminth infection and control · Parasite Biology and Host Interactions · Parasites and Host Interactions