Indole-3-carboxamide alleviates LPS-induced endometritis through suppressing ferroptosis and inflammation via regulating Aryl hydrocarbon receptor
Feng Chen, Rujin Cheng, Xiangyang Chen, Zhiheng Guo
TL;DR
Indole-3-carboxamide reduces inflammation and tissue damage in a mouse model of endometritis by targeting the AhR-SLC7A11 pathway.
Contribution
This study reveals a novel anti-inflammatory mechanism of I3A through suppression of ferroptosis and AhR activation in endometritis.
Findings
I3A reduced LPS-induced uterine injury and inflammation in mice.
I3A inhibited ferroptosis and NF-κB activation in the endometrium.
AhR inhibition reversed the protective effects of I3A, confirming its role in the mechanism.
Abstract
Indole-3-carboxamide (I3A), a derivative of tryptophan indole, has been reported to have anti-inflammatory role. This study aims to investigate the effects of I3A on LPS-induced endometritis in mice. The mice endometritis model was established and I3A was administered to mice orally (150 mg/kg/day) for two days. TNF-α and IL-1β production were analyzed by ELISA. The protein expression was measured by western blot. The pathological changes of mouse uterine tissue were observed by H&E staining. The experimental results showed I3A significantly alleviated LPS-induced uterine pathological injury. I3A treatment obviously attenuated LPS-induced MPO activity, TNF-α and IL-1β production. I3A also inhibited LPS-induced ferroptosis and NF-κB activation. Furthermore, I3A could up-regulate the expression of AhR and SLC7A11. The protective role of I3A on LPS-induced endometritis was reversed by…
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Taxonomy
TopicsPregnancy and preeclampsia studies · Reproductive System and Pregnancy · Ferroptosis and cancer prognosis