# HusMorph: a simple machine learning app for automated morphometric landmarking

## TL;DR

HusMorph is a user-friendly app that uses machine learning to automatically measure animal features from photos with high accuracy.

## Contribution

HusMorph introduces an accessible GUI-based tool for automated morphometric landmarking with high accuracy for non-experts.

## Key findings

- HusMorph achieved ~99.5% accuracy in measuring zebrafish length compared to manual measurements.
- The app includes a scale bar detection feature to convert pixel measurements to metric units.
- It was validated on 1935 standardized zebrafish photos.

## Abstract

Manually obtaining the length and other morphometric features of an animal can be time-consuming, and consistent measurements are challenging with large datasets. By leveraging high-throughput computing power and machine learning–based computer vision, such phenotypic data can be rapidly collected with high accuracy. Here we present HusMorph, a novel application with a simple and intuitive graphical user interface (GUI), based on the same machine learning method used in other pipelines such as ML-morph. It consists of an all-in-one package with the goal of making machine learning easy to use for non-experts. The user starts by setting any number of landmarks on a set of photos captured with a standardized setup. From this set, a machine learning model is generated by automatically and randomly searching for the best performing parameters. Next, the user can apply the model to predict landmarks on new standardized photos and visually confirm and export the results of the predictions. For measuring length between landmarks, an additional feature allows for detecting a scale bar for each photo to convert the length from pixels to a metric unit. Our application has been validated and applied to extract standard length from 1935 photos of zebrafish and performs with ~99.5% accuracy compared to manual measurements.