# Region-specific genomic variation and functional divergence of Helicobacter pylori clinical isolates from the gastric antrum and corpus

## TL;DR

This study shows how Helicobacter pylori strains from different parts of the stomach have unique genetic traits that affect their ability to cause disease.

## Contribution

The study reveals region-specific genomic and functional differences in H. pylori strains from the antrum and corpus of the stomach.

## Key findings

- Genetic heterogeneity was observed between antral and corpus isolates in adhesion-related genes and virulence factors.
- Corpus strains showed stronger adhesion and pro-inflammatory effects, while antral strains had higher vacuolating activity.
- The findings highlight niche-specific adaptations of H. pylori in different gastric regions.

## Abstract

The genetic variability of Helicobacter pylori contributes to differences in the severity of gastrointestinal diseases. Within the stomach, H. pylori exhibits diverse strain patterns and genetic variations that enable the evolution of new virulence factors, development of antibiotic resistance, and evasion of the host immune system. However, the comprehensive analysis of whole-genome sequences and their functional impact on gastric epithelial cells remains limited. In this study, we performed whole-genome sequencing, de novo assembly, and comparative genomic analysis on two pairs of H. pylori strains (v225/v226 and v290/v291) isolated from the antrum and corpus of two peptic ulcer patients. Bioinformatic tools were used to annotate and compare genes related to adhesion and virulence. Functional assays were conducted to assess strain-specific pathogenic effects on gastric epithelial cells. The analyses revealed substantial genetic heterogeneity between antral and corpus isolates, particularly in adhesion-related genes (sabA, babA/B), the cytotoxin-associated gene pathogenicity island (cag-PAI) cluster, and vacA sequences. Functional assays demonstrated region-specific differences, with corpus strains showing stronger adhesion and pro-inflammatory responses, whereas antral strains exhibited higher vacuolating activity. These findings demonstrate the ability of H. pylori to colonize specific stomach regions, undergo genetic diversification, and evolve niche-specific adaptations and pathogenicity in different gastric environments.

Helicobacter pylori is a major cause of severe gastrointestinal diseases. It can establish persistent colonization in different regions of the stomach, where distinct environmental conditions drive niche-specific adaptation. Here, we found that H. pylori evolves genetic diversity in various factors, including virulence factors, adhesion molecules, and outer membrane proteins, to facilitate persistent colonization. Understanding how H. pylori generates genetic diversity to support colonization is crucial for developing more effective infection management strategies, improving molecular detection, and refining personalized treatment approaches.