# Application of RAPD markers for Cuscuta species identification and biodiversity

## TL;DR

This study uses RAPD markers to identify and assess genetic diversity in Cuscuta species, showing their usefulness for species identification and understanding genetic patterns.

## Contribution

The novel use of RAPD markers for identifying Cuscuta species and analyzing their genetic diversity is demonstrated.

## Key findings

- RAPD markers effectively distinguish Cuscuta species even without reproductive organs.
- Native Cuscuta species show higher genetic diversity compared to C. campestris.
- Seed dispersal mechanisms influence genetic diversity more than geographic location.

## Abstract

Cuscuta species (dodders) are parasitic plants that harm both native and cultivated flora, presenting significant ecological and economic challenges. This study employs random amplified polymorphic DNA (RAPD) markers to evaluate genetic variation among 70 specimens collected from various localities across Bulgaria. The findings reveal notable differences in RAPD profiles between species, indicating that RAPD markers are effective for species identification, particularly when reproductive organs are absent. While C.
campestris exhibited a relatively uniform genetic profile across different populations, native species showed greater genetic heterogeneity, likely due to their development in diverse habitats. Geographical patterns were also observed in the genetic clustering of C.
campestris, although seed dispersal mechanisms appeared to play a larger role in shaping genetic diversity than geographic distribution alone. This research highlights the potential of RAPD markers for improving species identification and for understanding the genetic dynamics of Cuscuta populations, which is crucial for managing their spread and minimizing their agricultural impact.