# Beyond potency: A proposed lexicon for sensory differentiation of Cannabis sativa L. aroma

**Authors:** Solomon E. Isaacson, Adrianne R. Wilson-Poe, Tingting Ye, Yanping L. Qian, Thomas H. Shellhammer

PMC · DOI: 10.1371/journal.pone.0335125 · PLOS One · 2025-10-21

## TL;DR

This study creates a standardized way to describe the aroma of Cannabis sativa L., showing that chemical profiles alone don't fully explain sensory perception.

## Contribution

The study introduces a 25-term aroma lexicon for Cannabis and highlights the limitations of using chemical composition to predict sensory qualities.

## Key findings

- A 25-term aroma lexicon effectively differentiates Cannabis samples based on orthonasal aroma.
- Type I Cannabis is more often described as skunky and animalic, while Type III is more citrus and fruity.
- Terpene and volatile sulfur compound profiles poorly predict sensory aroma perception.

## Abstract

Aroma is a critical factor in consumer-perceived quality of Cannabis sativa L., yet standardized tools for describing the aromatic diversity of uncombusted Cannabis inflorescence are lacking. This study generated and evaluated a descriptive aroma lexicon for intact Cannabis inflorescence consisting of 25 terms with defined reference standards. A human panel evaluated 91 samples using a Check-All-That-Apply method. Multivariate analyses demonstrated the lexicon’s ability to differentiate samples based on orthonasal aroma. Type I and III Cannabis exhibited overlapping sensory profiles, though type I (high THC, low CBD) was more frequently described as skunky, musty, and animalic, whereas type III (low THC, high CBD) had higher frequencies of citrus, fruity, and candy-like aromas. Terpene profiling revealed clear chemical clusters, but terpene profiles alone poorly predicted sensory character. Terpinolene was the only compound consistently associated with sensory descriptors, specifically citrus and chemical. In type III samples, 43 volatile sulfur compounds were detected via gas chromatography with a pulsed flame photometric detector, including dimethyl sulfide, methional, and dimethyl trisulfide while others were tentatively identified or novel. However, neither terpene nor volatile sulfur compound profiles strongly predicted sensory perception. These results emphasize the limitations of chemical composition as a proxy for aroma quality. This work establishes a foundation for future research linking aroma, chemistry, and consumer preferences, and supports the development of quality metrics beyond delta-9-tetrahydrocannabinol potency.

## Linked entities

- **Chemicals:** delta-9-tetrahydrocannabinol (PubChem CID 2978), terpinolene (PubChem CID 11463), dimethyl sulfide (PubChem CID 1068), methional (PubChem CID 18635), dimethyl trisulfide (PubChem CID 19310)

## Full-text entities

- **Chemicals:** CBD (-), methional (MESH:C008390), sulfur compound (MESH:D013457), Terpene (MESH:D013729), dimethyl trisulfide (MESH:C054170), sulfur (MESH:D013455), THC (MESH:D013759), Terpinolene (MESH:C027009), dimethyl sulfide (MESH:C004784)
- **Species:** Cannabis sativa (species) [taxon 3483], Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12539713/full.md

## References

45 references — full list in the complete paper: https://tomesphere.com/paper/PMC12539713/full.md

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Source: https://tomesphere.com/paper/PMC12539713