# Navigating the Hepatic Immune Landscape With Fine Needle Aspiration of the Liver—An Emerging Technique

## TL;DR

Fine needle aspiration of the liver is a safe and effective way to study immune cells in liver diseases like PSC and steatotic liver disease.

## Contribution

Demonstrates that liver FNA is a safe and effective alternative to liver explants for immune profiling in non-infectious liver diseases.

## Key findings

- Liver FNA showed enrichment of CD69+ T and NK cells compared to peripheral blood.
- CXCR6+ NK, CD8+ central memory T, and MAIT cells were enriched in liver samples.
- Hepatic immune cells showed increased cytokine production and signaling compared to PBMCs.

## Abstract

Hepatic immune cell analysis is critical for understanding chronic inflammatory liver diseases, such as primary sclerosing cholangitis (PSC) and steatotic liver disease. However, liver immunoprofiling is limited due to reliance on end‐stage disease liver explants. Fine needle aspiration (FNA) is a minimally invasive technique that can overcome these limitations. We evaluate the safety and efficacy of liver FNA to profile hepatic immune subsets in non‐infectious liver conditions.

Flow cytometry and single‐cell RNA sequencing (scRNA‐seq) were used to compare the hepatic immune cell composition and gene expression to that of matched peripheral blood mononuclear cells (PBMCs).

We obtained liver FNAs from 38 patients. The median pain score was 0. No serious adverse effects were reported. Flow cytometry demonstrated enrichment of CD69+ T and natural killer (NK) cells in the liver (all P

adj
 < 0.05). ScRNA‐seq of 38 012 hepatic immune cells and 78 751 PBMCs in a patient subset showed specific enrichment of CXCR6
+ NK, CD8+ central memory T, and mucosal‐associated invariant T (MAIT) cells in the liver, and relatively lower CD4+ regulatory T cell (Treg) abundance (all P

adj
 < 0.05). Gene expression and cell–cell interaction analyses revealed increases in cytokine production, signalling, and responsiveness in hepatic immune cells compared to PBMCs.

FNA sampling is a safe approach for investigating the inflammatory landscape of PSC and other liver diseases. Single‐cell profiling reveals that FNAs capture tissue‐specific immune cell types and gene expression differences, suggesting this sampling method may provide a basis for future experimental medicine analyses.