# Effect of 35% hydrogen peroxide in promoting enamel whitening with orthodontic attachments bonded on its surface

**Authors:** Bruna Caroline Tomé BARRETO, Luiza Trindade VILELA, Guido Artemio MARAÑÓN-VÁSQUEZ, Matheus Melo PITHON, Margareth Maria Gomes de SOUZA

PMC · DOI: 10.1590/2177-6709.30.3.e252547.oar · Dental Press Journal of Orthodontics · 2025-10-20

## TL;DR

This study shows that 35% hydrogen peroxide can whiten teeth even when orthodontic attachments are bonded to the enamel surface.

## Contribution

Demonstrates that enamel whitening is effective with orthodontic attachments in place using 35% hydrogen peroxide.

## Key findings

- 35% hydrogen peroxide promotes enamel whitening regardless of orthodontic attachments.
- Both whitening and attachments independently affect color parameters on the CIELAB scale.
- The whitening effect is not hindered by the presence of orthodontic attachments.

## Abstract

The demand for aesthetics is high and the desire for white teeth during orthodontic treatment is a desire of patients.

To evaluate the effect of 35% hydrogen peroxide in promoting enamel whitening with orthodontic attachments bonded to its surface.

80 bovine incisors randomly divided into 4 groups (n=20): (GI) control group, (GII) group that received only tooth whitening, (GIII) group that received only bonding of attachments on the surface and (GIV) group that received bonding of attachments and whitening. Whitening was carried out following the manufacturer’s instructions while the attachments were bonded to the dental surface using a template after acid conditioning of the enamel and application of an adhesive system. Color evaluation was performed according to the LAB color scale of the Commission Internationale de l’Eclairage. Statistical analysis was performed using Jamovi program adopting a 5% significance level. Descriptive data analysis was performed, characterizing all study variables. General linear models adjusted by ordinary least squares regression were used to test the effect of whitening, enhancement use, and the interaction of both factors.

Both “whitening” and the use of “attachments” have an effect on all parameters of the CIELAB scale (keeping the other variable constant). The whitening effect was independent of the presence of attachments.

35% hydrogen peroxide is capable of promoting enamel whitening with orthodontic attachments bonded to its surface.

Despite being in vitro research, the study provides patients and professionals with the possibility of teeth whitening during orthodontic treatment with aligners.

## Linked entities

- **Chemicals:** hydrogen peroxide (PubChem CID 784)

## Full-text entities

- **Chemicals:** hydrogen peroxide (MESH:D006861)
- **Species:** Bos taurus (bovine, species) [taxon 9913], Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

4 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12536979/full.md

## References

33 references — full list in the complete paper: https://tomesphere.com/paper/PMC12536979/full.md

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Source: https://tomesphere.com/paper/PMC12536979