# Stage-specific fecal and salivary microbiota signatures for noninvasive detection of colorectal cancer

## TL;DR

This study identifies unique microbial patterns in feces and saliva that can help detect colorectal cancer at different stages without invasive procedures.

## Contribution

The study reveals stage-specific microbial signatures in fecal and salivary microbiota for noninvasive CRC detection.

## Key findings

- Fecal and salivary microbiota composition changes during CRC progression.
- Fecal microbial ASVs outperformed salivary ASVs in stage-specific CRC diagnosis.
- Salivary microbiota became progressively enriched in the gut as CRC progressed.

## Abstract

Fecal and salivary microbiota dysbiosis play a crucial role in the pathogenesis of colorectal cancer (CRC). We investigated whether the fecal and salivary microbiota were altered during colorectal tumorigenesis and evaluated their diagnostic performance.

We enrolled 30 metastatic CRC patients, 30 nonmetastatic CRC patients, and 30 healthy controls between October 2023 and September 2024. Fecal and salivary samples were collected for microbial profiling via 16S rDNA sequencing and bioinformatics analysis.

Fecal and salivary microbiota composition differed during CRC progression, with salivary microbiota progressively enriched in the gut. In addition, fecal and salivary microbial co-occurrence networks dynamically altered during CRC progression. The natural connectivity of fecal microbial community networks exhibited decreased stability, whereas salivary microbial community networks showed increased stability as CRC progressed. Finally, specific fecal microbial amplicon sequence variants (ASVs) associated with colorectal carcinogenesis enabled precise stage-specific diagnosis of CRC, outperforming salivary ASVs classifiers.

This study elucidates stage-specific microbial dynamics in CRC, providing novel insights into clinical diagnostic strategies.