# Nonsurgical Retreatment of a 16 × 9 mm Osteolytic Lesion With Buccal Bone Regeneration Using a Bioceramic Sealer: A Clinical and NGS-Based Microbiological Case Report With 30-Month Follow-Up

## TL;DR

A large bone lesion near the front teeth was successfully treated without surgery using a new type of sealer, with full healing observed over 30 months.

## Contribution

Demonstrates the effectiveness of a nonsurgical approach using a bioceramic sealer for treating large osteolytic lesions.

## Key findings

- The bioceramic sealer (CeraSeal) facilitated healing of a 16 × 9 mm lesion over 30 months.
- Microbiological analysis showed distinct bacterial profiles in affected teeth before treatment.
- Combining manual, rotary, and reciprocating protocols improved treatment outcomes.

## Abstract

This case report described the complete resolution of a large osteolytic lesion following a nonsurgical endodontic retreatment using a premixed bioceramic sealer. A 30-year-old healthy patient presented a large radiographic osteolytic lesion of 16 × 9 mm in correspondence with Teeth #42, #41, and #31. Clinical examinations revealed positive percussion tests and increased mobility of all incisors. The microbiological profile of the root canals was assessed through paper point sample collection, DNA extraction, and next-generation sequencing, revealing markedly different proportions of bacteria genera in the affected teeth. A secondary endodontic treatment was then performed, combining manual, rotary, and reciprocating protocols. A premixed bioceramic-based sealer (CeraSeal) was used for obturation due to the presence of wide and wet apexes. Radiographic (x-rays and CBCT) and clinical evaluations reported the progressive healing of the lesion over 30 months. This outcome supports the use of conservative treatments as a valid alternative to endodontic surgery for the management of extensive periapical lesions.