# A genome-wide identification and expression analysis of the class III peroxidase gene family in Mangifera indica under abiotic stresses and the MiPRX27 gene regulates oxidative stress

## TL;DR

This study identifies 76 peroxidase genes in mango and finds that one gene, MiPRX27, helps plants tolerate oxidative stress.

## Contribution

The study provides the first genome-wide analysis of class III peroxidase genes in mango and identifies MiPRX27's role in stress tolerance.

## Key findings

- 76 MiPRX genes were identified and unevenly distributed across mango chromosomes.
- MiPRX27 overexpression in Arabidopsis improved root growth under oxidative stress.
- MiPRX genes show differential expression under oxidative, drought, and salt stress.

## Abstract

Class III peroxidases (PRXs) are plant-specific enzymes that play vital roles in various physiological processes. However, the functional roles of mango PRXs under stress conditions remain poorly understood. In this study, we identified 76 MiPRX genes, which are unevenly distributed across the mango chromosomes. RT-qPCR analysis revealed differential expression of most MiPRX genes under oxidative, drought, and salt stress conditions, with MiPRX27 showing a particularly prominent role. Under oxidative stress, heterologous overexpression of MiPRX27 in Arabidopsis enhanced lateral root formation and accelerated root growth, suggesting that MiPRX27 contributes to reducing plant sensitivity to oxidative stress. Overall, this study provides a theoretical foundation for further exploration of MiPRX-mediated mechanisms underlying mango stress tolerance.