Comprehensive Analysis of Miscanthus NF-YA Genes Reveals Potential Involvement in Drought Stress Adaptation

TL;DR

This study identifies a gene in Miscanthus that helps the plant tolerate drought, offering potential for breeding more resilient crops.

Contribution

The study reveals the role of MsNF-YA4 in drought tolerance and its potential for crop improvement.

Findings

MsNF-YA4 is significantly upregulated under dehydration and osmotic stress in Miscanthus.

Overexpression of MsNF-YA4 in Arabidopsis enhances drought tolerance and activates stress-related genes.

MsNF-YA4 improves promoter activity of antioxidant and stress-related genes in a transient system.

Abstract

Miscanthus, a perennial grass, is renowned for its remarkable tolerance to abiotic stress. Excessive levels of drought severely impair plant growth and yield. Plant nuclear factor Y (NF-Y) transcription factors (TFs) play pivotal roles in regulating responses to drought stress in species such as Arabidopsis and maize. However, their functional roles in conferring drought tolerance in Miscanthus remain largely unexplored. This study’s genome-wide analysis and gene expression profiling of Miscanthus under dehydration/osmotic stress identified a transcription factors gene, MsNF-YA4, which was significantly upregulated under dehydration/osmotic stress. MsNF-YA4 overexpression in Arabidopsis significantly enhanced drought tolerance, leading to increased transcription of stress- and antioxidant enzyme-related genes. Compared with the wild type (WT), the transgenic lines exhibited markedly higher relative water content (RWC), chlorophyll content, proline level, and antioxidant enzyme activity. Furthermore, the MsNF-YA4/MsNF-YB3/MsNF-YC2 improved the transactivation of the Miscanthus P5CS1, SOD (Cu/Zn) and CAT1 promoters in the transient system. These results offer fresh perspectives on the role of Miscanthus NF-YAs in drought tolerance and offer promising genetic resources for developing drought-tolerant crops through breeding programs.

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