Pulsed Electric Fields Reshape the Malting Barley Metabolome: Insights from UHPLC-HRMS/MS
Adam Behner, Nela Prusova, Marcel Karabin, Lukas Jelinek, Jana Hajslova, Milena Stranska
TL;DR
This study explores how pulsed electric fields affect the chemical composition of barley during malting, revealing changes that may improve processing and safety.
Contribution
The study provides novel insights into metabolomic changes in barley after PEF treatment, identifying potential biomarkers linked to abiotic stress response.
Findings
PEF treatment significantly altered the metabolome of malting barley.
Identified biomarkers suggest a potential abiotic stress response in treated barley.
PEF may enhance enzymatic activity during malting, as indicated by biomarker profiles.
Abstract
The Pulsed Electric Field (PEF) technique represents a modern technology for treating and processing food and agricultural raw materials. The application of high-voltage electric pulses has been shown to modify macrostructure, improve extractability, and enhance the microbial safety of the treated matrix. In this study, we investigated metabolomic changes occurring during the individual technological steps of malting following PEF treatment. Methanolic extracts of technological intermediates of malting barley were analyzed using metabolomic fingerprinting performed with UHPLC-HRMS/MS. For data processing and interpretation, the freely available MS-DIAL—MS-CleanR—MS-Finder software platform was used. The metabolomes of the treated and untreated barley samples revealed significant changes. Tentatively identified PEF-related biomarkers included 1,2-diacylglycerol-3-phosphates,…
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Taxonomy
TopicsMicrobial Inactivation Methods · Radiation Effects and Dosimetry · Identification and Quantification in Food