# In Vitro Evaluation of Antimicrobial Effects of Endodontic Irrigants Containing Disodium Edetate and Chlorhexidine Gluconate, Octenidine Dihydrochloride, and Benzalkonium Bromide Against Intracanal Enterococcus faecalis

## TL;DR

This study tested how well different endodontic irrigants kill Enterococcus faecalis bacteria in root canals in a lab setting.

## Contribution

The study introduces and evaluates a novel irrigant containing disodium edetate and octenidine for antimicrobial efficacy.

## Key findings

- Endoxal, Octenisolv, and Endosal showed significant antimicrobial activity against Enterococcus faecalis.
- The novel irrigant containing disodium edetate and octenidine performed well in vitro.
- Further clinical studies are needed to confirm the efficacy of these irrigants.

## Abstract

Background/Objectives: The objective of this in vitro study was to compare and evaluate the in vitro antimicrobial effectiveness of Endosal, Octenisolv, and Endoxal against intracanal Enterococcus faecalis. Methods: The study sample consisted of 84 extracted single-rooted human teeth, which were divided into seven groups (12 roots in each group): Group 1—Endoxal, Group 2—Octenisolv, Group 3—Endosal, Group 4—15% ethylenediaminetetraacetic acid (EDTA), Group 5—2% sodium hypochlorite (NaOCl), Group 6—0.9% sterile saline solution (NaCl), and one positive control group where no irrigant was used. The roots were sterilized within an autoclave for 30 min at 121 °C and then contaminated with E. faecalis bacteria, after instrumentation and removing the smear layer from canals. The root canals were irrigated using a side-vented needle, and then ISO size 40 H-file was used to obtain fine dentine chips. Aliquots taken from the canals were plated on blood agar broth and the plates were incubated for 36 h. Results: In this study, significant differences were observed between the antimicrobial activity of Endoxal, Octenisolv, Endosal, 2% NaOCl, and sterile saline solution. Conclusions: The compound irrigants Endosal, Endoxal, and a novel irrigant containing disodium edetate and octenidine, which were evaluated in this study, exhibited relatively good antimicrobial properties against Enterococcus faecalis. The use of Endosal, Octenisolv or Endoxal appears promising, yet their clinical efficacy remains to be confirmed through further studies.