Foamable pluroleosomes system loaded with amlodipine as a repurposed antibacterial topical formulation against MRSA-induced infection; optimization, in-vitro, ex-vivo, and in-vivo studies
Alaa S. Eita, Amna M.A. Makky, Asem Anter, Islam A. Khalil
TL;DR
This study develops a foam-based drug delivery system using amlodipine to treat MRSA infections, showing promising in vitro and in vivo antibacterial effects.
Contribution
A novel foamable pluroleosome system for amlodipine is developed and optimized for topical antibacterial applications.
Findings
The optimized AML-PLOs formulation showed 71.25% drug entrapment and a controlled release over 48 hours.
The foam system demonstrated effective antibacterial activity against MRSA in both in vitro and in vivo models.
Confocal microscopy confirmed efficient penetration of the drug through skin layers.
Abstract
Amlodipine besylate (AML) is a renowned antihypertensive drug currently acknowledged for having antibacterial activity. AML repositioning can be helpful in the defeat of microbial-resistant strains. Loading amlodipine in the pluroleosomes (PLOs) foam system is desired to approach innovative remedies with a convenient application capable of targeting deep infections. The mixture design was employed to generate different pluroleosomes formulations consisting of various ratios of Pluronic F-127, oleic acid, and soya lecithin loaded with amlodipine. Based on the desirability function, the selected optimized formula (AML-PLOs), consisting of 4.875 for lecithin, one for oleic acid, and 1.125 for pluronic, exhibits a particle size of 320.56 ± 15.5 nm, a polydispersity index of 0.4461 ± 0.03, a surface charge of 15.261 ± 0.62 mV, and AML entrapment of 71.25 ± 3.52 %. The morphological image…
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Taxonomy
TopicsAntimicrobial agents and applications · Streptococcal Infections and Treatments · Advanced Drug Delivery Systems