Hofmeister-Driven Ion Pairing in Monovalent Salts Directs Fibrinogen Nanofiber Assembly during Drying
Stephani Stamboroski, Aparna Sai Malisetty, Kwasi Boateng, Jana Lierath, Jonas Aniol, Peter Schiffels, Paul-Ludwig Michael Noeske, Lucio Colombi Ciacchi, Susan Köppen, Dorothea Brüggemann
TL;DR
This study shows how different salts influence the formation of fibrinogen nanofibers during drying, offering insights for tissue engineering.
Contribution
The paper introduces a two-dimensional Hofmeister series to explain salt-driven fibrillogenesis via ion pairing.
Findings
KCl and NaCl produce smoother morphologies compared to Na-PO4 and K-PO4, which yield fibrous structures.
Kosmotropic anions like sulfate promote fiber formation, while chaotropic anions like oxalate do not.
Molecular dynamics simulations suggest ion-specific interactions at the fibrinogen/water interface.
Abstract
Fibrinogen nanofiber scaffolds hold promise for tissue engineering and wound healing due to their similarity to fibrin clots. We studied how alkaline salts (Na+, K+) influence fibrinogen precipitation during drying of highly saline dispersions. In situ roughness (Aq) monitoring revealed coprecipitation of salts and fibrinogen. SEM and Aq mapping showed morphologies from smooth (KCl) and faintly fibrous (NaCl) to highly rough and finely fibrous (Na-PO4, K-PO4). FTIR indicated that secondary structure changes are not always linked to fiber formation. XPS showed a stronger Na+ uptake, especially with fiber-forming salts. With Na+ and oxygen-containing polyvalent anions, kosmotropic SO4 2 – induced fibers, while chaotropic oxalate yielded smooth films. Mg2 + or K+ with SO4 2 – did not form any fibers. Molecular dynamics simulations suggest ion-specific binding at the fibrinogen/water…
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Taxonomy
TopicsBlood properties and coagulation · Erythrocyte Function and Pathophysiology · Electrohydrodynamics and Fluid Dynamics