Refined cuff technique minimizes surgical complexity in rat lung transplantation and improves outcomes
Mehmet Furkan Sahin, Gunes Esendaglı, Fatma Yıldırım, Erdal Yekeler, Banu Kılıcaslan
TL;DR
A refined surgical technique for rat lung transplants reduces complexity and improves long-term outcomes, making it easier to study chronic lung graft issues.
Contribution
A novel surgical model with intraoperative modifications and ventilation strategies that simplifies rat lung transplantation and improves reproducibility.
Findings
The refined technique achieved high procedural success and reproducible long-term survival in rat lung transplants.
Histopathological analysis showed chronic remodeling and reduced acute rejection with the new method.
The technique uses accessible clinical-grade materials and has a short learning curve.
Abstract
Orthotopic lung transplantation in rats serves as a pivotal platform for exploring long-term allograft outcomes, yet technical complexity has limited its widespread application. Here, we present a refined surgical model that combines structured intraoperative modifications with tailored mechanical ventilation strategies to ensure procedural consistency and biologic fidelity. Key interventions—such as a custom leak-proof endotracheal tube, a temporally controlled reperfusion maneuver, and an optimized anastomotic sequence—collectively addressed critical challenges including airway obstruction, graft impaired perfusion, and venous fragility. The protocol demonstrated a high procedural success rate and reproducible long-term survival, with histopathological analysis confirming progressive chronic remodeling and diminished acute rejection. Importantly, the technique demonstrated features…
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Taxonomy
TopicsTransplantation: Methods and Outcomes · Organ Transplantation Techniques and Outcomes · Respiratory Support and Mechanisms