# Staphylococcus aureus transcriptomics and single-cell sequencing approaches

## TL;DR

This paper reviews how genomics, transcriptomics, and single-cell sequencing have improved understanding of Staphylococcus aureus, a dangerous antibiotic-resistant bacterium.

## Contribution

The paper provides a comprehensive review of transcriptomics and single-cell sequencing applications in S. aureus research.

## Key findings

- Transcriptomics has significantly advanced understanding of S. aureus biology and pathogenesis.
- Single-cell sequencing is emerging as a powerful tool for studying S. aureus heterogeneity.
- Genomics approaches have revealed insights into S. aureus as a commensal and pathogen.

## Abstract

Staphylococcus aureus is an important cause of human infections globally and ranks among the top causes of death by bacteria. In addition, the microbe is notorious for developing resistance to antibiotics. Methicillin-resistant S. aureus is endemic in healthcare facilities and the community in many regions of the world. Although our understanding of S. aureus as a human commensal organism and opportunistic pathogen remains incomplete, the use of genomics and transcriptomics approaches for S. aureus research has advanced this knowledge significantly over the past 20 years. This article reviews genomics approaches, with special emphasis on transcriptomics and single-cell sequencing, used to study S. aureus, past and present, and highlights selected discoveries made with these methods and new applications moving forward.