# Immune cell isolation from lymphoid and nonlymphoid organs

**Authors:** Jong Seok Park, Yoontae Lee

PMC · DOI: 10.1016/j.mocell.2025.100277 · 2025-09-19

## TL;DR

This editorial provides practical protocols for isolating immune cells from both lymphoid and nonlymphoid organs.

## Contribution

The paper introduces concise protocols for immune cell isolation from diverse organs, including nonlymphoid tissues.

## Key findings

- Immune cells are found in both lymphoid and nonlymphoid organs.
- Protocols are provided for isolating immune cells from various tissues.
- These cells play roles in local immune surveillance and response.

## Abstract

Immune cells are distributed across various tissues. While a majority are concentrated in primary and secondary lymphoid organs such as the bone marrow, thymus, lymph nodes, and spleen, a subset resides in nonlymphoid organs, including the kidney, liver, and lung, as well as the peritoneal cavity, where they play critical roles in local immune surveillance and response. In this editorial, we outline concise and practical protocols for the isolation of immune cells from a range of lymphoid and nonlymphoid organs.

## Full-text entities

- **Diseases:** TISSUES (MESH:D017695), ISOLATION (MESH:C565377), bleeding (MESH:D006470), IMMUNE CELL (OMIM:252500), IMMUNE (MESH:D007154)
- **Chemicals:** salt (MESH:D012492), Percoll (MESH:C016039), BioRender (-), penicillin (MESH:D010406), NH4Cl (MESH:D000643), EDTA (MESH:D004492), streptomycin (MESH:D013307), KHCO3 (MESH:C026329), PBS (MESH:D007854)
- **Species:** Mus musculus (house mouse, species) [taxon 10090]

## Figures

2 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12519291/full.md

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Source: https://tomesphere.com/paper/PMC12519291