# Micro-hardness of root canal dentin using various irrigating solutions: An in vitro study

**Authors:** Richa Pathak, Meetu Mathur, Diganta Kumar Borah, Amit Kumar, Sonam Agrawal, Chinmayee Prusty, Pratik Surana

PMC · DOI: 10.6026/973206300211057 · Bioinformation · 2025-05-31

## TL;DR

This study compares how different root canal irrigation solutions affect the hardness of dentin in extracted teeth.

## Contribution

The study identifies 2% Chlorhexidine as a suitable irrigation solution due to its minimal impact on dentin microhardness.

## Key findings

- 17% EDTA caused the most significant reduction in dentin microhardness.
- 2% Chlorhexidine did not negatively affect dentin microhardness.
- 3% Sodium Hypochlorite and 2% Chlorhexidine showed no significant difference compared to the control.

## Abstract

Evaluation of changes in dentin microhardness during root canal irrigation using eighty extracted mandibular premolars is of interest.
After decoronation and halving, baseline microhardness was measured with a Vickers tester. The samples were divided into four groups:
control (Normal Saline), 3% Sodium Hypochlorite, 17% EDTA, and 2% Chlorhexidine Gluconate, with each immersed in their respective
irrigants for 15 minutes at 37°C. Results showed decreased microhardness in all groups except the control, with 17% EDTA causing the
most significant reduction. Notable differences were found between groups 2, 4, and 1, but not between 1 and 3. Consequently, 2%
Chlorhexidine is considered a suitable irrigation solution as it did not negatively impact dentin microhardness.

## Linked entities

- **Chemicals:** Sodium Hypochlorite (PubChem CID 23665760), EDTA (PubChem CID 6049), Chlorhexidine Gluconate (PubChem CID 9552081), Normal Saline (PubChem CID 5234)

## Full-text entities

- **Chemicals:** EDTA (MESH:D004492), Sodium Hypochlorite (MESH:D012973), Chlorhexidine (MESH:D002710), Chlorhexidine Gluconate (MESH:C010882), Saline (MESH:D012965)

## Full text

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## References

17 references — full list in the complete paper: https://tomesphere.com/paper/PMC12357693/full.md

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Source: https://tomesphere.com/paper/PMC12357693