# Urinary Bladder Matrix as a Guide Bone Regeneration Barrier Membrane for Inhibiting Cell Invasion and Promoting Bone Formation

## TL;DR

A new bone regeneration membrane made from urinary bladder matrix improves bone growth and prevents unwanted cell invasion in dental implants.

## Contribution

A novel urinary bladder matrix-coated membrane enhances GBR performance with better mechanical strength and slower degradation.

## Key findings

- The UBM-SIS membrane showed superior tensile strength and slower degradation than Bio-Gide.
- In canine models, UBM-SIS increased bone trabeculae quantity and maturity over 24 weeks.
- The membrane resisted fibroblast invasion and promoted osteoblast growth in early stages.

## Abstract

Natural resorbable
collagen membranes are widely used in guided
bone regeneration (GBR) in oral implantology and prosthodontics. However,
the rapid degradation and inadequate mechanical properties raise concerns
about potentially compromising the ultimate bone regeneration efficacy.
In this study, we developed a novel GBR membrane by coating a urinary
bladder matrix (UBM) onto small intestinal submucosa (SIS), a commonly
used acellular material, to improve the cell-barrier and bone-regeneration
performances. The results showed that the UBM-SIS membrane exhibited
superior tensile strength, high compliance, and slower degradation
rate compared to a commercial Bio-Gide membrane, thereby enhancing
the support and maintenance properties. In vitro studies indicated
enhanced osteogenic behavior and higher osteogenic cytokine expression
in human bone marrow-derived mesenchymal stromal cells (hBMSCs) cultured
with UBM-SIS extract. In canine mandibular defect models, we proved
that the UBM layer effectively resisted fibroblast invasion in the
early stage, thereby enhancing the bone meal duration and osteoblast
growth. Microcomputed tomography analysis revealed the greater quantity
and maturity of bone trabeculae using a UBM-SIS membrane, exhibiting
enhanced bone formation at 12 weeks and trabecular maturity at 24
weeks. In conclusion, we proposed a modified resorbable GBR membrane
that may improve clinical outcomes in prosthodontics.