# Complete genome sequence of mycobacteriophage Hashim76, a B13 subcluster phage, from Hanahan, South Carolina

## TL;DR

This paper reports the complete genome sequence of a mycobacteriophage named Hashim76, isolated from mud in South Carolina.

## Contribution

The study provides a new genome sequence for a B13 subcluster phage and identifies its genetic features and similarity to another phage.

## Key findings

- Hashim76 has a circular double-stranded DNA genome of 70,230 bp with a GC content of 70.1%.
- The genome contains 101 putative protein-coding genes but lacks tRNA sequences.
- Hashim76's genome shows high similarity (93.91% identity) to the phage Zenteno07.

## Abstract

Hashim76, a B13 subcluster bacteriophage extracted from mud (Hanahan, South Carolina), infects Mycobacterium smegmatis mc2155 and exhibits siphovirus morphology. It has a circular double-stranded DNA genome (70,230 bp; GC content = 70.1) containing 101 putative protein-coding genes but no tRNA sequences. Whole-genome BLASTn alignment revealed high similarity to Zenteno07 (93.91% identity, 90% query coverage).