# Complete genome sequences of Mycobacterium smegmatis mc2155 cluster E bacteriophages Policronamos and Palpatine **Authors:** Shule M. Aggabao, Rebecca Hassell, Bryson S. Leary, Sheena I. Bautista, Hayzen H. Chamberlain, Kyla Radke, Harry M. Peless, Payson C. Danielson, Matthew N. Jackson, Hyunbi Hwang, Jacob D. Scott, Elisa A. Correa Lazaro, Atalie B. Bogh, Jayden S. Longhurst, Spencer T. Payne, Parker Danielson, Natalie A. Olsen, Christopher C. Harrell, Austin M. Johnson, Thomas Wilhite, Jeffrey K. Schachterle, Staci Avery, Donald P. Breakwell, Brett E. Pickett PMC · DOI: 10.1128/mra.00392-25 · Microbiology Resource Announcements · 2025-07-23 ## TL;DR This paper reports the complete genome sequences of two bacteriophages, Policronamos and Palpatine, which infect the Mycobacterium smegmatis mc2155 strain. ## Contribution The study provides new genomic data and ORF annotations for two mycobacteriophages in subcluster E. ## Key findings - Policronamos has a 75,894-bp genome with 146 open reading frames. - Palpatine has a 75,809-bp genome with 140 open reading frames. - Both phages show gene synteny matching other subcluster E mycobacteriophages. ## Abstract Mycobacteriophages Policronamos and Palpatine are tailed phages infecting the Mycobacterium smegmatis mc2155 strain. The genome for Policronamos is 75,894 bp long with 146 open reading frames (ORFs), while the Palpatine genome is 75,809 bp long with 140 ORFs. The gene synteny of both mycobacteriophages closely matches other phage genomes in subcluster E. ## Full-text entities - **Species:** Mycolicibacterium smegmatis MC2 155 (strain) [taxon 246196] ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12352092/full.md ## Figures 1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12352092/full.md ## References 13 references — full list in the complete paper: https://tomesphere.com/paper/PMC12352092/full.md --- Source: https://tomesphere.com/paper/PMC12352092