# Isolation and genome annotation of Rowlf, a novel cluster EG bacteriophage with a purple acid phosphatase-like gene

**Authors:** Emily Sullivan, Ninahazwe Mireill, Aisley B. Allen, Phoenix R. Larsen, Daniel Puentes Navarro, Madison P. Renn, Trenton W. Shappee, Madalyne M. Sisk, Alexander W. Thomas, Giselle Trejo, Lexy Bellew, Lee George, Sariah Hepworth, Savannah Webb, Sara Sadeghi, Joshua Burger, Eva Githuku, Jack F. Shurley, Anna S. Grinath, Michael A. Thomas

PMC · DOI: 10.1128/mra.00314-25 · Microbiology Resource Announcements · 2025-07-22

## TL;DR

A new bacteriophage named Rowlf was isolated and its genome was annotated, revealing a gene similar to purple acid phosphatase found in plants and bacteria.

## Contribution

The discovery and genome annotation of a novel cluster EG bacteriophage with a unique phosphatase-like gene.

## Key findings

- Rowlf is a siphovirus with a 62,044 bp genome containing 104 putative genes.
- The genome includes a gene resembling purple acid phosphatase, typically found in leguminous plants and bacteria.
- Rowlf was classified into cluster EG based on similarity to actinobacteriophages.

## Abstract

Isolated from grass thatch in Pocatello, Idaho, Microbacterium foliorum phage Rowlf has a siphovirus morphology and is presumed to be a lytic bacteriophage. Rowlf has a 62,044 bp genome with 104 putative genes, including a purple acid phosphatase-like gene found in leguminous plants and bacteria. Based on similarity to actinobacteriophages, Rowlf was assigned cluster EG.

## Linked entities

- **Species:** Microbacterium foliorum (taxon 104336)

## Full-text entities

- **Species:** Bacteriophage sp. (species) [taxon 38018], Microbacterium foliorum (species) [taxon 104336]

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12352085/full.md

## References

20 references — full list in the complete paper: https://tomesphere.com/paper/PMC12352085/full.md

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Source: https://tomesphere.com/paper/PMC12352085