# Report of longan witches’ broom-associated virus in lychee imported from China

## TL;DR

A virus associated with longan witches' broom was found in lychee seedlings imported from China.

## Contribution

The complete sequence of a LWBaV isolate from lychee seedlings was determined.

## Key findings

- The virus was identified in post-entry quarantine samples of lychee seedlings.
- RNA sequencing, RT-PCR, and Sanger sequencing were used to determine the complete sequence of a large single polyprotein.

## Abstract

The complete sequence of a large single polyprotein of a longan witches’ broom-associated virus (LWBaV) isolate, obtained from post-entry quarantine samples of lychee seedlings from China, was determined by RNA sequencing, RT-PCR amplification, and Sanger sequencing.