# Complete genome annotation of the cluster EC Microbacterium phage Nicole72 from Pittsburgh, Pennsylvania

**Authors:** Adam J. Ziegler, Christa P. Joby, Hams A. Kamil, Ethan Johnson, Ashlyn L. Ivey, Vanessa A. Fullante, Gautam Ghosh, Laura Leal Martinez, Gabrielle A. Tutelo, Devya Wilson, Christine A. Byrum

PMC · DOI: 10.1128/mra.00507-25 · Microbiology Resource Announcements · 2025-07-22

## TL;DR

This paper reports the full genome analysis of a new microbacteriophage, Nicole72, isolated in Pittsburgh.

## Contribution

The study provides a complete genome annotation of the newly identified phage Nicole72.

## Key findings

- Nicole72 has a 55,431 bp genome with 90 predicted protein-coding genes.
- The phage belongs to the siphovirus morphology and is closely related to the Megan phage with 81.5% nucleotide identity.

## Abstract

Nicole72, a microbacteriophage isolated from a flowerbed in Pittsburgh, Pennsylvania, has a 55,431 bp genome containing 90 predicted protein-coding genes and no transfer RNAs or transfer-messenger RNAs. Nicole72 has siphovirus morphology and is most closely related to the EC cluster microbacteriophage Megan where BLASTn indicates 81.5% identity, 86% query coverage.

## Linked entities

- **Species:** Microbacterium (taxon 33882)

## Full-text entities

- **Diseases:** EC (MESH:D005955)
- **Chemicals:** queuosine (MESH:D009704), CaCl2 (MESH:D002122), 7H9 (-), agar (MESH:D000362), copper (MESH:D003300), uranyl acetate (MESH:C005460), archaeosine (MESH:C081066)
- **Species:** Bacteriophage sp. (species) [taxon 38018], Microbacterium (genus) [taxon 33882]
- **Cell lines:** Nicole72 — Canis lupus familiaris (Dog), Canine neoplasm, Cancer cell line (CVCL_3453)

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12352058/full.md

## References

20 references — full list in the complete paper: https://tomesphere.com/paper/PMC12352058/full.md

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Source: https://tomesphere.com/paper/PMC12352058