# Differential inflammatory responses to acute exercise and ex vivo immune challenge in young and master athletes

## TL;DR

This study compares how young and older athletes respond to inflammation after exercise, finding that young athletes have stronger immune reactions while older athletes show more controlled responses.

## Contribution

The study provides new insights into how aging and exercise influence inflammatory responses to immune challenges.

## Key findings

- Young athletes showed higher IL-6 and TNF-α release in whole blood after exercise.
- Master athletes had lower LPS-induced TNF-α release, increasing only after exercise.
- Young athletes exhibited stronger cytokine responses under PMA/ionomycin stimulation compared to master athletes.

## Abstract

Lifelong exercise is associated with beneficial immune adaptations, but the extent to which these adaptations manifest during an acute inflammatory challenge remains unclear. Therefore, we aimed to compare the inflammatory responses to ex vivo whole blood and peripheral blood mononuclear cells [PBMCs] cultures from young and master athletes, before and after a single bout of moderate-intensity exercise.

Young (n=7; 22 ± 4 years) and master (n=12; 52 ± 9 years) female and male athletes with similar performance levels performed a 30-minute bout of moderate-intensity exercise. Blood samples were collected before and post-exercise to assess cytokine production in whole blood and PBMCs after stimulation with lipopolysaccharide [LPS] and a cocktail with phorbol 12-myristate 13-acetate [PMA] plus ionomycin.

In whole blood, LPS induced higher interleukin [IL]-6 release in both groups, with a greater increase in young athletes at post-exercise (p=0.014). Tumor necrosis factor [TNF]-α levels increased only in young athletes (p<0.0001). In PBMCs, master athletes showed lower LPS-induced TNF-α release, increasing only post-exercise (p<0.034), whereas young athletes responded at both baseline (p<0.001) and post-exercise (p=0.003). Under PMA/ionomycin stimulation, TNF-α (p<0.0001) and interferon (IFN)-γ (p=0.007) release increased only in young athletes, while IL-6 production decreased in young athletes at baseline (p=0.002) and post-exercise (p=0.003).

Young athletes exhibit a stronger cytokine response to ex vivo inflammatory stimuli, while master athletes demonstrate a more controlled and regulated inflammatory profile.