# Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV)

## TL;DR

This paper introduces new TaqMan RT-qPCR methods to detect two harmful viruses in strawberries, enabling early and accurate identification to prevent outbreaks.

## Contribution

The study establishes singleplex and duplex TaqMan RT-qPCR systems for detecting SMoV and SVBV in strawberries.

## Key findings

- TaqMan RT-qPCR systems enable accurate detection of SMoV and SVBV even at low viral concentrations.
- A duplex assay allows simultaneous quantification of both viruses in a single reaction.
- The methods improve detection efficiency and support virus risk assessment in strawberry cultivation.

## Abstract

SMoV and SVBV are two major viruses that pose significant threats to the global strawberry industry. Both are latent viruses, making early detection difficult due to their uneven distribution and low concentration in host tissues. Traditional RT-PCR techniques are insufficient for precise and quantitative detection. In this study, TaqMan RT-qPCR detection systems for SMoV and SVBV were established for application in practical production settings, enabling accurate, rapid, and efficient detection of strawberry viruses. When viral accumulation in plants is low, the highly sensitive TaqMan RT-qPCR technique allows for accurate quantification, facilitating the early identification of infected plants and preventing large-scale outbreaks in cultivation areas. The development of a duplex TaqMan RT-qPCR assay enables simultaneous quantification of SMoV and SVBV in a single reaction, improving detection efficiency and providing technical support for risk assessment and effective control of strawberry viral diseases.