Exogenous Sucrose Improves the Vigor of Aged Safflower Seeds by Mediating Fatty Acid Metabolism and Glycometabolism

TL;DR

Exogenous sucrose helps revive aged safflower seeds by boosting fatty acid and sugar metabolism, not just by providing energy.

Contribution

The study reveals that exogenous sucrose enhances germination in aged safflower seeds through specific metabolic pathways.

Findings

Exogenous sucrose increases soluble sugars and fatty acids in aged safflower seeds.

Sucrose treatment up-regulates key enzymes and genes in fatty acid and sugar metabolism pathways.

Glyoxylate recycler and oil body interactions are enhanced, promoting oil body degradation.

Abstract

Safflower (Carthamus tinctorius L.) seeds, rich in triacylglycerols, have poor fatty acid-to-sugar conversion during storage, affecting longevity and vigor. Previous experiments have shown that the aging of safflower seeds is mainly related to the impairment of energy metabolism pathways such as glycolysis, fatty acid degradation, and the tricarboxylic acid cycle. The treatment with exogenous sucrose can partially promote the germination of aged seeds. However, the specific pathways through which exogenous sucrose promotes the germination of aged safflower seeds have not yet been elucidated. This study aimed to explore the molecular mechanism by which exogenous sucrose enhances the vitality of aged seeds. Phenotypically, it promoted germination and seedling establishment in CDT-aged seeds but not in unaged ones. Biochemical analyses revealed increased soluble sugars and fatty acids in aged seeds with sucrose treatment. Enzyme activity and transcriptome sequencing showed up-regulation of key enzymes and genes in related metabolic pathways in aged seeds, not in unaged ones. qPCR confirmed up-regulation of genes for triacylglycerol and fatty acid-to-sugar conversion. Transmission electron microscopy showed a stronger connection between the glyoxylate recycler and oil bodies, accelerating oil body degradation. In conclusion, our research shows that exogenous sucrose promotes aged safflower seed germination by facilitating triacylglycerol hydrolysis, fatty acid conversion, and glycometabolism, rather than simply serving as a source of energy to supplement the energy deficiency of aged seeds. These findings offer practical insights for aged seeds, especially offering an effective solution to the aging problem of seeds with high oil content.

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