# Behavioral and Proteomics Studies on the Regulation of Response Speed in Mice by Whey Protein Hydrolysate Intervention

## TL;DR

This study shows that whey protein hydrolysate can improve mice's response speed to stimuli, possibly by affecting brain metabolism and receptor interactions.

## Contribution

The novel contribution is the identification of proteomic changes linked to improved response speed following whey protein hydrolysate intervention.

## Key findings

- Whey protein hydrolysate improved response speed in mice to thermal and mechanical stimuli.
- Proteomic analysis revealed altered proteins related to arginine, proline metabolism, and neuroreceptor interactions in the hippocampus.
- Findings suggest a neuromodulatory role for whey protein hydrolysate in enhancing cognitive and response performance.

## Abstract

Background: Response speed refers to an individual’s ability to perceive and react to harmful stimuli, which can vary due to genetics, neural regulation, and environmental factors. Our previous study demonstrated that whey protein hydrolysate was a potential means to enhance cognitive function. Methods: This study used a variety of behavioral methods to evaluate the functional effects of whey protein hydrolysate on improving reaction speed, and revealed its potential mechanisms through proteomics analysis. Results: The results showed that whey protein hydrolysate improved response speed in mice when tested against thermal pain, mechanical strength stimuli, and prepulse inhibition. Proteomic analysis of the hippocampus revealed changes in proteins related to arginine and proline metabolism, as well as neuroactive ligand–receptor interactions. Conclusions: These findings provide new insights into the neuromodulatory effects of whey protein hydrolysate and support its potential role in enhancing response speed and cognitive performance.