# Dietary Supplementation with Probiotics Alleviates Intestinal Injury in LPS-Challenged Piglets

## TL;DR

Adding probiotics to piglets' diets helps protect their intestines from LPS-induced damage and improves immune responses.

## Contribution

The study demonstrates that specific probiotics can mitigate LPS-induced intestinal injury in piglets through immune modulation.

## Key findings

- Probiotic supplementation reduced LPS-induced intestinal damage and improved barrier function.
- Probiotics modulated immune responses by affecting NF-κB, IL-4, IL-6, IL-10, and other gene expressions.
- Both Lactobacillus and Bacillus probiotics showed health-promoting effects in LPS-challenged piglets.

## Abstract

This study aimed to assess whether dietary supplementation with probiotics could alleviate intestinal injury in lipopolysaccharide (LPS)-challenged piglets. Healthy weaned piglets were randomly allocated to four individual groups (n = 6): (1) a control group; (2) an LPS group; (3) an LPS + Lactobacillus group; and (4) an LPS + Bacillus group. The control and LPS groups received a basal diet, while the probiotic groups were provided with the same basal diet supplemented with 6 × 106 cfu/g of Lactobacillus casei (L. casei) or a combination of Bacillus subtilis (B. subtilis) and Bacillus licheniformis (B. licheniformis) at a dosage of 3 × 106 cfu/g, respectively. On day 31 of the trial, overnight-fasted piglets were killed following the administration of either LPS or 0.9% NaCl solution. Blood samples and intestinal tissues were obtained for further analysis several hours later. The results indicate that dietary supplementation with probiotics significantly exhibited health-promoting effects compared with the control group and effectively reduced LPS-induced histomorphological damage to the small intestine, impairments in barrier function, and dysregulated immune responses via modulation of enzyme activity and the expression of relevant genes, such as nuclear factor-kappa B (NF-κB), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 10 (IL-10), claudin-1, nuclear-associatedantigenki-67 (Ki-67), and β-defensins-1 (pBD-1). Collectively, these results suggest that dietary supplementation with probiotics could alleviate LPS-induced intestinal injury by enhancing the immunity and anti-inflammatory responses in piglets. Our research provides a theoretical basis for the rational application of probiotics in the future.