Lycopene Inhibits PRRSV Replication by Suppressing ROS Production

TL;DR

Lycopene, a natural compound, inhibits PRRSV virus replication by reducing harmful oxygen molecules and inflammation in pig cells.

Contribution

This study reveals that lycopene suppresses PRRSV replication by targeting ROS production and inflammation.

Findings

Lycopene does not harm cell viability, cycle progression, or apoptosis.

Lycopene inhibits PRRSV replication by suppressing reactive oxygen species (ROS) production.

Lycopene reduces PRRSV-induced inflammation in infected cells.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV), an enveloped single-stranded positive-sense RNA virus, poses a significant threat to global swine production. Despite the availability of modified live virus and inactivated vaccines, their limited efficacy and safety concerns highlight the urgent need for novel antiviral therapeutics. This study aimed to investigate the molecular mechanisms by which lycopene inhibits PRRSV replication. Initial assessments confirmed that lycopene did not adversely affect cellular viability, cell cycle progression, or apoptosis. Using fluorescence microscopy, flow cytometry, immunoblotting, quantitative real-time PCR (qRT-PCR), and viral titration assays, lycopene was shown to exhibit potent antiviral activity against PRRSV. Mechanistic studies revealed that lycopene suppresses reactive oxygen species (ROS) production, which is critical for PRRSV proliferation. Additionally, lycopene attenuated PRRSV-induced inflammatory responses, as demonstrated by immunoblotting, ELISA, and qRT-PCR assays. These findings suggest that lycopene inhibits PRRSV replication by modulating ROS levels and mitigating inflammation, offering a promising avenue for the development of antiviral therapeutics. This study provides new insights and strategies for combating PRRSV infections, emphasizing the potential of lycopene as a safe and effective antiviral agent.

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