# Effects of Different Packaging on the Purine Content and Key Enzymes of Refrigerated Yellow Croaker (Larimichthys crocea)

## TL;DR

This study shows that modified atmosphere packaging slows purine metabolism in refrigerated yellow croakers, preserving quality better than air or vacuum packaging.

## Contribution

Modified atmosphere packaging (CO2/N2: 80/20) was shown to effectively regulate purine metabolism and enzyme activity in refrigerated fish.

## Key findings

- Modified atmosphere packaging significantly reduced hypoxanthine accumulation compared to air packaging.
- Modified atmosphere packaging delayed ATP degradation and maintained higher IMP content for longer.
- Xanthine oxidase and purine nucleoside phosphorylase activities were lower in modified atmosphere packaging.

## Abstract

In this study, we investigated the effects of air packaging, vacuum packaging and modified atmosphere packaging (CO2/N2: 80/20) on the purine metabolism and enzyme activities of refrigerated large yellow croakers. The results showed that modified atmosphere packaging significantly inhibited microbial growth, delayed adenosine triphosphate degradation and maintained higher IMP content (1.93 μmol/g on day 21) compared to the air packaging group (2.82 μmol/g on day 12). The total purine content increased with storage time, with hypoxanthine content increasing significantly and occupying most of the total content, which was the key factor for the elevation of purine, followed by adenine content showing a significant decreasing trend. Hypoxanthine accumulation was significantly suppressed in the modified atmosphere packaging group (2.31 μmol/g on day 18), which was much lower than that in the air packaging group (5.64 μmol/g), whereas xanthine and guanine did not show significant differences among the groups. The key enzymes xanthine oxidase and purine nucleoside phosphorylase were much less active in modified atmosphere packaging, effectively delaying the cascade reaction of inosine monophosphate → hypoxanthine → xanthine. The study confirmed that modified atmosphere packaging intervenes in purine metabolism through enzyme activity regulation, providing a theoretical basis for the preservation of low purine aquatic products.