# Key Factors Influencing Bacillus cereus Contamination in Hot Ready-to-Eat Meal Delivery

**Authors:** Tomáš Komprda, Olga Cwiková, Vojtěch Kumbár, Gabriela Franke, Petr Kouřil, Ondřej Patloka, Josef Kameník, Marta Dušková, Alena Zouharová

PMC · DOI: 10.3390/foods14152605 · Foods · 2025-07-24

## TL;DR

This study examines how meal types and packaging affect the risk of Bacillus cereus contamination during hot meal delivery.

## Contribution

The study identifies packaging materials as key factors influencing bacterial growth during food delivery.

## Key findings

- PP trays and PP boxes delayed temperature decrease, reducing B. cereus growth risk.
- Bacillus cereus counts remained below unsafe levels after four hours of simulated delivery.
- Meal type had no significant effect on temperature decrease or bacterial growth.

## Abstract

With increasing popularity of food delivery services, the microbial safety of transported meals should be ensured. An effect of the type of a meal (cooked rice; mashed potatoes; mushroom sauce), inner primary packaging (sugarcane bagasse [SB] tray; polypropylene [PP] tray), secondary container (polyester/polyethylene foam/aluminum foil [PPA] bag; PP box) on the time interval of the internal hot ready-to-eat (RTE) meal temperature decrease to the value critical for Bacillus cereus growth (40 °C) was tested during a simulated delivery; in aliquot samples of the same meals, B. cereus growth was quantified presuming a natural contamination of the meals. Type of a meal had no effect on the tested time interval (p > 0.05). Packaging a meal in the PP tray as compared to the SB tray and inserting primary trays into the PP box instead of PPA bag delayed (p < 0.05) the internal meal temperature decrease by 50 and 15 min, respectively. Average B. cereus counts in the naturally contaminated meals after the four-hour culturing at 40 °C was 2.99 log CFU·g−1. It was concluded that a hot RTE meal delivered up to four hours under the tested conditions is not likely to facilitate B. cereus growth above unacceptable levels.

## Linked entities

- **Species:** Bacillus cereus (taxon 1396)

## Full-text entities

- **Chemicals:** PPA (-), polyethylene (MESH:D020959), polyester (MESH:D011091)
- **Species:** Oryza sativa (Asian cultivated rice, species) [taxon 4530], Bacillus cereus (species) [taxon 1396], Solanum tuberosum (potatoes, species) [taxon 4113]

## Full text

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## Figures

6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12346577/full.md

## References

52 references — full list in the complete paper: https://tomesphere.com/paper/PMC12346577/full.md

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Source: https://tomesphere.com/paper/PMC12346577