# Andrographis paniculata Extract Supports Skin Homeostasis by Enhancing Epidermal Stem Cell Function and Reinforcing Their Extracellular Niche

## TL;DR

Andrographis paniculata extract improves skin health by boosting stem cell function and strengthening the skin's structural support.

## Contribution

The study reveals a novel role for Andrographis paniculata extract in enhancing epidermal stem cell function and extracellular matrix stability.

## Key findings

- Andrographis paniculata extract increases Collagen IV and Laminin production while decreasing MMP-9 expression in skin models.
- The extract preserves stem cell potential by upregulating markers like collagen XVII, β1-integrin, Ki-67, KRT15, Survivin, and LRIG1.
- APE promotes Laminin production in keratinocyte stem cells under UVB-induced stress, suggesting protection against photo-damage.

## Abstract

Skin aging is characterized by compromised epidermal homeostasis and dermo-epidermal junction (DEJ) integrity, resulting in reduced stem cell potential and impaired tissue regeneration. This study investigated the effects of Andrographis paniculata extract (APE) on keratinocyte stem cells (KSCs) and DEJ composition in human skin. Using human skin explants and cell culture models, we demonstrated that APE treatment enhances DEJ composition by increasing Collagen IV and Laminin production while decreasing MMP-9 expression, without altering epidermal structure or differentiation. In the same model, APE preserved stemness potential by upregulating markers related to niche components (collagen XVII and β1-integrin), proliferation (Ki-67 and KRT15), and stem cell capacity (Survivin and LRIG1). In vitro studies revealed that APE selectively stimulated KSC proliferation without affecting transit amplifying cells and promoted Collagen IV and Laminin secretion, particularly in KSCs. Furthermore, in a co-culture model simulating a compromised DEJ (UVB-induced), APE increased Laminin production in KSCs, suggesting a protective effect against photo-damage. These findings indicate that APE enhances DEJ composition and preserves stem cell potential, highlighting its promise as a candidate for skin anti-aging strategies targeting stem cell maintenance and extracellular matrix stability to promote skin regeneration and repair.