# Disaccharides and Fructooligosaccharides (FOS) Production by Wild Yeasts Isolated from Agave

## TL;DR

This study explores how wild yeasts from agave can produce prebiotic fructooligosaccharides and disaccharides, which have potential industrial applications.

## Contribution

The study identifies specific yeasts capable of producing various FOS and disaccharides, and determines optimal production conditions using experimental design.

## Key findings

- Two yeasts, P. kudriavzevii ITMLB97 and C. lusitaniae ITMLB85, showed fructosyltransferase activity.
- FOS like 1-kestose, 6-kestose, and neokestose, along with disaccharides, were successfully produced.
- Optimal conditions for FOS and disaccharide production were identified using Box–Behnken designs.

## Abstract

Fructooligosaccharides (FOS) are short fructans with different degrees of polymerization (DP) and bonds in their structure, generated by the distinct activities of fructosyltransferase enzymes, which produce distinct types of links. FOS are in high demand on the market, mainly because of their prebiotic effects. In recent years, depending on the link type in the FOS structure, prebiotic activity has been shown to be increased. Studies on β-fructanofuranosidases (Ffasa), enzymes with fructosyltransferase activity in yeasts, have reported the production of 1F-FOS, 6F-FOS, and 6G-FOS. The aims of this investigation were to evaluate the capability of fifteen different yeasts to grow in Agave sp. juices and to determine the potential of these juices as substrates for FOS production. Additionally, the research aimed to corroborate and analyze the fructosyltransferase activity of enzymatic extracts obtained from agave yeasts by distinct induction media and to identify the role and optimal parameters (time and sucrose and glucose concentrations) for FOS and disaccharides production through Box–Behnken designs. To carry out such a task, different techniques were employed: FT-IR, TLC, and HPAEC-PAD. We found two yeasts with fructosyltransferase activity, P. kudriavzevii ITMLB97 and C. lusitaniae ITMLB85. In addition, within the most relevant results, the production of the FOS 1-kestose, 6-kestose, and neokestose, as well as disaccharides inulobiose, levanobiose, and blastose, molecules with potential applications, was determined. Overall, FOS production requires suitable yeast species, which grow in a medium under optimal conditions, from which microbial enzymes with industrial potential can be obtained.