# Effects of beer addition on the fermentation quality and flavor development of pickled peppers

**Authors:** Linzhu Li, Wei Su, Yingchun Mu, Yu Jiao, Ling Chen, Huayan Luo

PMC · DOI: 10.1016/j.fochx.2025.102845 · Food Chemistry: X · 2025-08-05

## TL;DR

Adding beer to pickled pepper fermentation changes flavor and texture by altering microbial and chemical profiles.

## Contribution

This study identifies how beer addition affects microbial communities and flavor compounds in pickled peppers.

## Key findings

- Beer addition increased esters, alcohols, and aldehydes, enhancing flavor.
- Lactiplantibacillus and Wickerhamomyces correlated with key volatile flavor compounds.
- Beer improved texture and sensory quality while altering pH and acidity levels.

## Abstract

This study explored the effects of beer as a fermentation medium additive, with beer addition levels of 25 % and 50 %, on the fermentation quality and flavor development of pickled peppers. Beer addition resulted in higher pH, reducing sugar, and nitrite levels, as well as improved textural crispness, while reducing total acidity and salinity. Microbial community analysis indicated that Firmicutes, Proteobacteria, and Ascomycota were the dominant phyla. Volatile compounds were predominantly categorized into esters, alcohols, aldehydes, and terpenes; collectively, 37 differential volatile compounds were identified, including 24 esters, 5 aldehydes, 3 alcohols, 3 terpenes, 1 acid, and 1 ketone. Moreover, Spearman correlation analysis revealed that Lactiplantibacillus and Wickerhamomyces exhibited significant correlations with key flavor substances. This study can provide a reference for optimizing the fermentation process of pickled peppers and improving the flavor quality of products.

•Flavor and microbial composition were studied during the fermentation of pickled peppers with added beer•The addition of beer increased the contents of esters, alcohols, and aldehydes.•Lactiplantibacillus and Wickerhamomyces were related to key volatile compounds.•The addition of beer improved instrumental textural crispness and sensory quality.

Flavor and microbial composition were studied during the fermentation of pickled peppers with added beer

The addition of beer increased the contents of esters, alcohols, and aldehydes.

Lactiplantibacillus and Wickerhamomyces were related to key volatile compounds.

The addition of beer improved instrumental textural crispness and sensory quality.

## Linked entities

- **Species:** Lactiplantibacillus (taxon 2767842), Wickerhamomyces (taxon 599737)

## Full-text entities

- **Diseases:** disease (MESH:D004194)
- **Chemicals:** Terpenes (MESH:D013729), Ethyl linoleate (MESH:C007678), alpha-terpineol (MESH:C016775), PEP (MESH:D010728), ethyl undecanoate (-), linalool (MESH:C018584), Aldehydes (MESH:D000447), ethyl acetate (MESH:C007650), phenols (MESH:D010636), lipid (MESH:D008055), isoamyl acetate (MESH:C020377), ethyl laurate (MESH:C007677), sulfanilic acid (MESH:D013425), cholesterol (MESH:D002784), azo compound (MESH:D001391), (+)-valencene (MESH:C506706), carbon (MESH:D002244), alkanes (MESH:D000473), methyl palmitate (MESH:C019012), wintergreen oil (MESH:C515973), acetyl-CoA (MESH:D000105), He (MESH:D006371), ethyl palmitate (MESH:C007680), Phenethyl alcohol (MESH:D010626), Acetic acids (MESH:D000085), isoamyl alcohol (MESH:C029683), phenylethylene (MESH:D020058), malic acid (MESH:C030298), 3,5-dinitrosalicylic acid (MESH:C027011), polytetrafluoroethylene (MESH:D011138), 1-pentanol (MESH:C024999), d-limonene (MESH:D000077222), Succinic acid (MESH:D019802), carbon dioxide (MESH:D002245), N-(1-naphthyl)ethylenediamine (MESH:C008588), TCA (MESH:D014238), hexanal (MESH:C010463), tricarboxylic acid (MESH:D014233), oxalic acid (MESH:D019815), carbohydrates (MESH:D002241), ketone (MESH:D007659), nonanal (MESH:C008664), glyceraldehyde 3-phosphate (MESH:D005986), beta-elemene (MESH:C445979), Glucose (MESH:D005947), sugar (MESH:D000073893), Nitrite (MESH:D009573), Pyruvate (MESH:D019289), methyl linoleate (MESH:C005575), phenylacetaldehyde (MESH:C013192), Phenylalanine (MESH:D010649), hexadecanal (MESH:C001647), methanol (MESH:D000432), Citric acid (MESH:D019343), acetic acid (MESH:D019342), alcohol (MESH:D000438), ethyl nonanoate (MESH:C000723071), Esters (MESH:D004952), ethanol (MESH:D000431), water (MESH:D014867)
- **Species:** Alternaria sect. Alternaria (section) [taxon 2499237], Colletotrichum (genus) [taxon 5455], Leuconostoc (genus) [taxon 1243], Enterovirus C (no rank) [taxon 138950], Rosenbergiella (genus) [taxon 1356488], Pseudomonadota (proteobacteria, phylum) [taxon 1224], Wickerhamomyces (genus) [taxon 599737], Cucumis sativus (cucumber, species) [taxon 3659], Debaryomyces (genus) [taxon 4958], Aspergillus (genus) [taxon 5052], Staphylococcus (genus) [taxon 1279], Lactobacillus (genus) [taxon 1578], Fungi (kingdom) [taxon 4751], Pseudomonas (RNA similarity group I, genus) [taxon 286], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Weissella (genus) [taxon 46255], Bacillota (clostridial firmicutes, phylum) [taxon 1239], Pantoea (genus) [taxon 53335], Xanthomonas (genus) [taxon 338], Kosakonia (genus) [taxon 1330547], Epicoccum (genus) [taxon 104397], Pichia (genus) [taxon 4919], Lactococcus (lactic streptococci, genus) [taxon 1357]

## Full text

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## Figures

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## References

40 references — full list in the complete paper: https://tomesphere.com/paper/PMC12345330/full.md

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Source: https://tomesphere.com/paper/PMC12345330