# Automatic phenotyping using exhaustive projection pursuit

## TL;DR

A new automated tool called EPP identifies cell populations in flow cytometry data by analyzing two-dimensional projections and optimizing gating regions.

## Contribution

EPP introduces an automated, statistically significant method for phenotyping in flow cytometry data using exhaustive projection pursuit.

## Key findings

- EPP identifies phenotypes through optimized two-dimensional projections and statistically significant gating regions.
- The tool was validated on four well-characterized flow cytometry datasets.
- EPP's C++ code is freely available and integrates with software like MATLAB and FlowJo.

## Abstract

One of the most common objectives in the analysis of flow cytometry data is the identification and delineation of phenotypes, distinct populations of cells with shared characteristics in the measurement dimensions. We have developed an automated tool to comprehensively identify these cell populations by Exhaustive Projection Pursuit (EPP). The method evaluates all two-dimensional projections among the suitable data dimensions and creates an optimized sequence of statistically significant gating regions that identify all phenotypes supported by the data. We evaluate the results of EPP on four well characterized data sets from the literature. The C++ code for EPP can be called from any computing environment. We illustrate this with a MATLAB utility that integrates EPP with FlowJo. All source code is freely available.

An automated tool (EPP) identifies distinct cell phenotypes in flow cytometry data through optimized 2D gating as shown in 4 published datasets. EPP’ C++ code integrates with any software, as demonstrated in MATLAB with FlowJo.