# Treats containing cannabidiol, L-tryptophan and α-casozepine have a mild stress-reducing effect in dogs

## TL;DR

This study found that treats containing cannabidiol, L-tryptophan, and α-casozepine may have a mild stress-reducing effect in dogs during car travel.

## Contribution

The study evaluates a combination of CBD with L-tryptophan and α-casozepine for stress reduction in dogs, showing a mild effect.

## Key findings

- CBD combined with L-tryptophan and α-casozepine reduced cortisol increase during stress in dogs.
- Higher CBD doses resulted in higher plasma CBD levels compared to lower doses.
- CBD plasma levels varied significantly between individual dogs.

## Abstract

Demand for stress-reducing products aimed at pets has risen in recent years, demonstrated by an ever-growing market of nutritional and odor-based products. Previous research has demonstrated an effect of Cannabidiol (CBD), L-tryptophan and α-casozepine on stress-related behaviours in a variety of animal species, including dogs. The objective of this study was to explore the efficacy of a treat product containing two different doses of CBD (2 mg/kg BW and 4 mg/kg BW) in addition to a 2mg/kg BW dose of CBD combined with L-Tryptophan and α-casozepine (blend) in comparison to a placebo.

A blinded cross-over study was performed in which 54 dogs received a single dose of each treatment two hours prior to exposure to a previously developed stress paradigm (10 min. car travel). A range of behavioural and physiological measures were collected pre/post (plasma CBD levels, serum cortisol) or during (heart rate, heart rate variability, surface temperature, activity, posture, stress-related behaviours, qualitative ratings) the stress paradigm.

All treatments resulted in elevated post-test CBD levels in the plasma in comparison to placebo (p < 0.001), the 4 mg/kg BW CBD had higher post-test CBD levels in comparison to the 2 mg/kg BW CBD without the blend (p = 0.002). Furthermore, the 2 mg/kg BW CBD combined with the blend treatment resulted in a significantly smaller increase in cortisol from baseline to post-stress (p = 0.016) in response to car travel in comparison to the placebo. However, no other significant effects of treatment were observed, and CBD plasma levels were highly variable between individual dogs, which may have impacted results.

CBD combined with the blend had a mild stress-reducing effect in dogs. Further exploration of the efficacy of CBD in reducing stress and anxiety, including interactions with different active ingredients and individual differences in absorption and metabolism are warranted.