# Faster efficacy and reduced nodule occurrence with PLLA (poly-l-lactic acid) porous microspheres

## TL;DR

A new form of PLLA microspheres shows faster results and fewer nodules in skin filler applications.

## Contribution

Structural modification of PLLA microspheres accelerates efficacy and reduces nodule formation.

## Key findings

- PLLA porous microspheres showed faster collagen deposition starting at week 2.
- The modified microspheres had more homogeneous tissue distribution and fewer microaggregates.
- Enhanced inflammatory cell infiltration was observed in the PLLA porous microsphere group.

## Abstract

Poly-L-lactic acid (PLLA) has gained prominence as an injectable dermal filler as it can both stimulate collagen regeneration and deliver long-lasting effects. However, its application is often hampered by delayed therapeutic onset and adverse events, particularly nodule formation, likely due to uneven distribution caused by the easy formation of small clumps during PLLA reconstitution.

In this study, PLLA porous microspheres were administered at six dorsal sites on one flank of New Zealand white rabbits, with six contralateral sites receiving Löviselle as control. At predetermined experimental time points, subjects were humanely euthanized. Post-euthanasia, tissue sections underwent histological examination through hematoxylin and eosin (H&E) staining and Masson’s trichrome technique, with subsequent statistical analysis of the observational data.

The PLLA porous microsphere group demonstrated significantly faster onset of action compared to the control, with observable collagen deposition as early as week 2, enhanced inflammatory cell infiltration, more homogeneous tissue distribution, and substantially fewer microaggregates upon histological examination.

Structural modification of PLLA microspheres has successfully accelerated their onset of action while reducing nodule formation, thereby providing novel insights for clinical applications.