# A Protein-Centric Mass Spectrometry Approach for Species Identification within Harmful Algal Blooms

**Authors:** Jaspreet K. Sound, Hannah E. Wedgwood, Qonita Afinanisa, Tim W. Overton, Aneika C. Leney

PMC · DOI: 10.1021/jacs.5c07419 · Journal of the American Chemical Society · 2025-07-28

## TL;DR

This paper introduces a new method using mass spectrometry to detect and monitor harmful algal blooms, specifically cyanobacteria, in lake samples.

## Contribution

The novelty lies in using native mass spectrometry for direct, rapid identification of cyanobacteria in environmental samples.

## Key findings

- Native mass spectrometry effectively detects cyanobacteria in lake samples before and during bloom formation.
- The method enables rapid characterization of cyanobacterial populations, providing insights into bloom dynamics.
- This approach supports the development of strategies to mitigate harmful algal blooms.

## Abstract

Harmful algal blooms present severe environmental threats,
impacting
water quality, aquatic ecosystems, and human health. The frequency
and intensity of these blooms are rising, largely driven by global
warming and changing climatic conditions. There is an urgent need
for innovative methods to monitor blue-green algae, also known as
cyanobacteria, to enable the implementation of preventative measures.
Here, we show that native mass spectrometry is an effective tool for
detecting cyanobacteria directly from lake samples, both prior and
during bloom formation. Our approach allows for the rapid characterization
of cyanobacterial populations within lakes, offering valuable insights
into the dynamics of cyanobacterial species associated with harmful
algae blooms. Overall, we highlight the exceptional capability of
native mass spectrometry in directly detecting and monitoring cyanobacterial
blooms, which will support the development of more effective strategies
to mitigate this growing environmental challenge.

## Full-text entities

- **Species:** Cyanobacteriota (blue-green algae, phylum) [taxon 1117], Homo sapiens (human, species) [taxon 9606], uncultured cyanobacterium (species) [taxon 1211], PX clade (clade) [taxon 569578]

## Full text

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## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12333325/full.md

## References

40 references — full list in the complete paper: https://tomesphere.com/paper/PMC12333325/full.md

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Source: https://tomesphere.com/paper/PMC12333325