# ZC3H13 mediates N6-methyladenosine modification of SNTB1 to promote epithelial-mesenchymal transition in gastric cancer

## TL;DR

This study shows that ZC3H13 promotes gastric cancer progression by modifying SNTB1 through m6A methylation, which enhances cancer cell spread.

## Contribution

The study is the first to report ZC3H13's role in gastric cancer via m6A modification of SNTB1, linking it to epithelial-mesenchymal transition.

## Key findings

- ZC3H13 is highly expressed in gastric cancer tissues and is associated with poor patient prognosis.
- ZC3H13 promotes cancer cell proliferation, invasion, and migration by inducing epithelial-mesenchymal transition.
- ZC3H13 regulates SNTB1 through YTHDF1-dependent m6A modification, leading to cancer progression.

## Abstract

Gastric cancer (GC) is one of the highly aggressive human malignant tumors. However, as one of the components of m6A methylation, ZC3H13 has not been reported to regulate the occurrence and development of GC. We determined the expression of ZC3H13 in GC and its correlation with prognosis using the TCGA and ACRG datasets. The effect of ZC3H13 on GC was elucidated through in vivo and in vitro assays. Next, we identified the downstream target of ZC3H13 via MeRIP-seq, and RNA-seq combined with multi-omics analysis, and explored the regulatory mechanism of ZC3H13 in GC using methods such as immunoprecipitation. We found that ZC3H13 is highly expressed in GC tissues and contributes to the poor prognosis of GC patients. ZC3H13 promoted the proliferation, invasion and migration of GC cells in vivo and in vitro, possibly by facilitating the EMT process. In addition, SNTB1 was identified as a downstream target of ZC3H13. ZC3H13-regulated YTHDF1-dependent m6A modification led to post-transcriptional activation of SNTB1 and induce phenotypic changes and EMT activation in GC cells. We determined that ZC3H13 is significantly upregulated in GC and promotes its progression. ZC3H13 mediated YTHDF1-dependent m6A modification of SNTB1 promotes the progression of GC by influencing the EMT process. Our study is the first to report the crucial role of ZC3H13 mediated m6A modification in GC, and we believe that ZC3H13 can serve as a potential therapeutic target for GC.