# Evaluation of calcium alginate hydrogel for suprachoroidal buckling in a rabbit model—a pilot study on efficacy and biocompatibility

## TL;DR

This pilot study shows that calcium alginate hydrogel is safe and effective for suprachoroidal buckling in rabbit eyes.

## Contribution

The study introduces calcium alginate hydrogel as a novel and biocompatible material for suprachoroidal buckling in a rabbit model.

## Key findings

- Calcium alginate hydrogel was successfully injected into the suprachoroidal space of 80.95% of rabbit eyes.
- The hydrogel maintained a suprachoroidal indentation for 3 months with no complications in most cases.
- Histopathological evaluation showed no abnormalities in lenses or retinas of treated eyes.

## Abstract

This study aimed to evaluate the therapeutic efficacy, safety, and biocompatibility of calcium alginate hydrogel in suprachoroidal buckling procedures performed on rabbit eyes.

A total of 21 healthy New Zealand White rabbits underwent surgery, during which calcium alginate hydrogel was injected into the suprachoroidal space of one eye. Monthly ophthalmic examinations were performed for 3 months following the procedure. The assessments included external eye and fundus examinations, color fundus imaging, and optical coherence tomography. At the end of the observation period, both eyes from each rabbit were enucleated and subjected to histopathological evaluation.

The surgery was successfully performed on 21 eyes, with a success rate of 80.95% (17/21). A total of 17 eyes showed favorable postoperative outcomes and no complications. The average maximum dome height is 2,470.42 ± 876.11 μm on the day of operation and 1,585.97 ± 351.93 μm 3 months postoperatively, with the average difference of 866.37 ± 592.94 μm. A total of four eyes experienced inadvertent perforations of both the choroid and retina, leading to vitreous and suprachoroidal hemorrhages. Histological evaluation confirmed the persistent presence of a suprachoroidal indentation in the treated eyes of the 17 rabbits without complications, with no observable abnormalities detected in the lenses or retinas.

Calcium alginate hydrogel proved to be a safe and effective material for suprachoroidal buckling in rabbits, exhibiting excellent biocompatibility.