# To Evaluate the Effect of Vitamin D on MicroRNAs in Polycystic Ovary Syndrome in Rat; An Animal Study: Effect of Vit-D on micro-RNAs in PCOS Rats

## TL;DR

This animal study shows that vitamin D affects certain microRNAs and long non-coding RNAs in polycystic ovary syndrome (PCOS) in rats, suggesting it may help manage the condition.

## Contribution

The study demonstrates vitamin D's regulatory effect on exosomal miRNAs and lncRNAs in PCOS, suggesting a novel therapeutic strategy.

## Key findings

- Vitamin D significantly increased miR-186 and miR-21 expression in PCOS rats.
- PCOS rats showed higher Lnc ROR and Lnc MALAT1 expression compared to the vitamin D group.
- Vitamin D had no significant effect on miR-29 expression in PCOS rats.

## Abstract

polycystic ovary syndrome (PCOS) is a metabolic disorder with menstrual and
ovulatory irregularities and elevated risk factors for reproductive diseases
that affects women in reproductive ages. A significant number of patients
with PCOS have insufficient levels of vitamin- D (Vit-D). This study aimed
to investigate the effect of Vit-D on the expression of micro-RNAs in PCOS
in rats.

24 female rats randomly were divided into four groups, including: 1)
Control., receiving no drug (testosterone enanthate or Vit-D), 2) Pos.,
receiving the solvent of testosterone enanthate and Vit-D (ethanol), 3)
induction of PCOS, receiving testosterone enanthate, and 4) Vit-D: treatment
with Vit-D. The blood serum, used for the extraction of miR-186, mir-29,
mir-21, Lnc ROR, Lnc MALA1, and H19 Lnc exosomes and the exosomes were
separated from the serum. Data analysis was conducted using the ANOVA
statistical test and SPSS software version 16 to examine significance at the
level of P0.05.

The results showed that the expression of miR-186 was significantly increased
in the Vit D group compared to the PCOS group (P0.01). Similarly, miR-21
expression was significantly higher in the Vit D group than in the PCOS
group (P0.001). However, no significant difference was observed in miR-29
expression between the Vit D and PCOS groups (P0.05). Additionally, the
expression of LncRNA H19 (P0.05), Lnc ROR (P0.001), and Lnc MALAT1 (P0.01)
was significantly higher in the PCOS group compared to the Vit D group.

These findings suggest that Vit-D plays a regulatory role in the expression
of exosomal miRNAs and lncRNAs involved in PCOS pathogenesis. Given its
potential to modulate genetic factors associated with PCOS, Vit-D
supplementation could be considered as a supportive therapeutic strategy for
managing PCOS. However, further studies are needed to explore its precise
molecular mechanisms and clinical implications.