# Epstein-Barr virus nuclear antigen 1 upregulates Derlin1 and PSMD10 expression in HeLa cells

## TL;DR

This study shows that the Epstein-Barr virus protein EBNA1 increases the expression of Derlin1 and PSMD10 in HeLa cells, which may contribute to cervical cancer development.

## Contribution

The study identifies EBNA1's specific upregulation of Derlin1 and PSMD10 in HeLa cells, linking it to potential cancer progression.

## Key findings

- EBNA1 significantly increased Derlin1 and PSMD10 gene expression in HeLa cells.
- No significant changes were observed in ZEB1 and CNN3 gene expression.
- The findings suggest a potential role for EBNA1 in promoting cervical cancer cell survival.

## Abstract

Background: Epstein-Barr Virus (EBV), a potent viral carcinogen, plays a crucial role in the development of various malignancies. Among its proteins, EBV nuclear antigen-1 (EBNA1) stands out for its ability to modulate gene expression. In this study, we explored the impact of EBNA1 on the expression patterns of four cellular genes—Derlin1, ZEB1, CNN3, and PSMD10—in HeLa cells.

Materials and Methods: Three distinct categories of HeLa cells were established:
EBNA1-Transfected Cells: These cells were transfected with the EBNA1 gene.Control Plasmid-Transfected Cells: These cells received transfection with a control plasmid.Non-Transfected Cells (Control Group): These cells were not subjected to any transfection.

EBNA1-Transfected Cells: These cells were transfected with the EBNA1 gene.

Control Plasmid-Transfected Cells: These cells received transfection with a control plasmid.

Non-Transfected Cells (Control Group): These cells were not subjected to any transfection.

After RNA extraction, we employed real-time PCR to evaluate the transcriptional levels of four specific genes—Derlin 1, ZEB1, CNN3, and PSMD10—in each of the three cell groups. The Mann-Whitney U-test was subsequently utilized to compare means, and statistical significance was determined based on p-values below 0.05. Data were meticulously recorded in an Excel 2016 spreadsheet.

Results: The results demonstrated that HeLa cells transfected with the EBNA1 plasmid exhibited significantly increased expression levels of Derlin1 (p = 0.028) and PSMD10 (p = 0.028) genes compared to cells transfected with the control plasmid. However, the expression changes observed in CNN3 and ZEB1 were not statistically significant (p = 0.99 and p = 0.2, respectively).

Conclusions: Our findings suggest that increase expression levels of Derlin1 and PSMD10 genes in HeLa cells by the EBV-EBNA1 might induce cancer cell survival and accelerates the development of cervical cancer (CC). However, to establish a conclusive link between EBV-EBNA1 and CC progression, further investigations are warranted.