# Ameliorative Effect of Heat-Killed Lactiplantibacillus plantarum Q1 (LPQ1) on Lipid Accumulation: Integration of Network Pharmacology with In Vitro and In Vivo Experiments

## TL;DR

This study shows that heat-killed Lactiplantibacillus plantarum Q1 helps reduce lipid accumulation by regulating key metabolic and inflammatory pathways.

## Contribution

The study integrates network pharmacology with in vitro and in vivo experiments to validate the therapeutic potential of LPQ1 for lipid metabolism disorders.

## Key findings

- LPQ1 modulates lipid metabolism by regulating PPAR-γ and TNF-α expression.
- Network pharmacology findings align with experimental results in both in vitro and in vivo models.
- LPQ1 shows potential as a therapeutic agent for lipid metabolism disorders.

## Abstract

Lipid metabolism disorders are expanding at an alarming rate worldwide pressuring the entire healthcare system. Therefore, exploring effective therapeutic treatments for lipid metabolism disorders is a hot spot in research. The therapeutic effects of paraprobiotics on obesity are still not fully explored through the integration of network pharmacology analysis with in vitro and in vivo experiments to confirm their beneficial effect. Therefore, the current study aimed to investigate the ameliorative effect of heat-killed Lactiplantibacillus plantarum Q1 (LPQ1) on lipid accumulation through the integration of network pharmacology analysis with in vitro and in vivo experiments. Findings of network pharmacology analysis conform with in vitro and in vivo experiments, revealing that the modulating effect of LPQ1 on lipid metabolism was effective through the regulation of PPAR-γ and TNF-α expression. Therefore, LPQ1 can be promoted as an effective therapeutic agent against lipid metabolism disorders owing to its modulatory effect on hepatic lipid metabolism and inflammation.